E-ISSN 2636-865X
Research Article
Demineralized Calf Vertebra Model: Can Be Used in Osteoporosis Research?
1 Department of Neurosurgery, Bozyaka Training and Research Hospital, Izmir, Turkey  
2 Department of Neurosurgery, Tepecik Training and Research Hospital, Izmir, Turkey  
3 Department of Pathology, Bozyaka Training and Research Hospital, Izmir, Turkey  
4 Departement of Biochemistry, Gaziemir State Hospital, Izmir, Turkey  
5 Department of Orthopedics, Hand Microsurgery Orthopaedy Traumatology Hospital, Izmir, Turkey  
Neurol Sci Neurophysiol 2014; 31: 680-686

Key Words: Demineralisation, osteoporosis, calf vertebrae
Abstract

In osteoporosis researchs, animal models have been used widely, but every model have associated with some advantages and disadvantages. In vitro studies with fresh cadaver look like good solution, however paucity of cadaver restricts its use. Demineralized calf vertebra model have been introduced as a new option. The aim of present study to check this new model on histopathological view and bone mineral density changes. Lumbar spines of seven fresh calves were cleaned from soft tissues and were submitted to DXA exams which provide the areal bone mineral density (BMD ) and bone mineral contents ( BMC ). Than, demineralization procedure was done for each L1 and L5 vertebras of calves whereas other vertebras were used as control group. Following procedure, new DEXA exams were made. Light microscobic evaluation with Hematoxylen-Eosine staining was achieved in control and study groups. As a result, bone mineral density changes was statistically significant between two group, but histological appearance was far from ordinary bone with empty bone lacunes in demineralized vertebras.

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